[jena]

Hi,

My Question:
For testing the purity of a protein which method is suitable?

a. FDNB(N-terminus sequencing)
b. Edman sequence
c. Both are suitable

Answer: c

Is this correct
Thank You

[sdekivit]

seems correct: the N-amino acid is identified with FDNB and Edman sequencing reveals the amino acid sequence of the protein.

[jena]

Hi,

What if after running and Edman sequence, you find that the number of proteins that you put in for the experiment gives for example, Lys-Lys-Gly-Glu, does this mean that it produced three proteins or just one

Thank You

[sdekivit]

Hi,

What if after running and Edman sequence, you find that the number of proteins that you put in for the experiment gives for example, Lys-Lys-Gly-Glu, does this mean that it produced three proteins or just one

Thank You

i can't follow you, because you 're talking about peptides and come up with amino acids in a particular sequence.