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Topic: Why do we use caffeine as an internal standard in hplc?  (Read 2046 times)

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Offline Moz29386

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Why do we use caffeine as an internal standard in hplc?
« on: October 23, 2011, 04:46:03 PM »
Hi guys just trying to find out the answer to the above question as I couldnt find it.

Also which compounds are more polar (in order):

Theophyline

Theobromine and

Caffeine?

Thanks!!
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Offline Honclbrif

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Re: Why do we use caffeine as an internal standard in hplc?
« Reply #1 on: October 23, 2011, 05:23:33 PM »
1) Depends on why you're doing the HPLC

2) Look up the structures
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