[Google01]

How glycerine presence is determined  in a mixture by simple analytical methods? (qualitative or quantitative)

And, how Sorbitol presence is determined by analytical methods?

[Stepan]

Depends on what what kind of range we are talking about: ppm? %? 100%?  What are other components?

[Google01]

range is approximately %10. the other components are micro emulsions of silicone polymers (polysiloxane) a little bit nonionic surfactant and mostly water.(glyserine)

For sorbitol, other component is just cellulase enzyme. and water.

[Stepan]

If in both solutions glycerol and sorbitol are the only dissolved components and the rest is water, you can build correlation between glycerol (sorbitol) concentration and solution property.

First separate solids by filtration or centrifugation. Measure a) refractive index; b) specific gravity. From your data and a correlation chart determine concentration of  analyte.

For Refractive Index measurement you need around 0.2 mL of solution and working range would be from 0.5 to 100%.
For Specific Gravity measurement you need around 100 mL of solution and working range would be from 1 to 100%.

[Google01]

Thank you for your answer but this doesnt help me.

i need to know about "presence of glycerol"  this means the mixture may contain it or NOT! this situation changes everything. amount of it is secondary important.

Refractive index is clearly unusable for mixtures. Other components can mask it.

[Stepan]

The answer was based on data you provided. At 10% concentration of target analyte and micro impurities of siloxanes and enzymes in water the macro methods usually work. You should expect +/-1% absolute error. Those are the techniques used in good processing and their systems are as complex as yours. 

If you do not have access to TLC, GC (glycerol), or HPLC-RI, try wet chemistry. I believe there are qualitative reactions on polyols. The one I remember from high-school chemistry is reaction with copper hydroxide in ammonia hydroxide solution.  Probably there are more sensitive techniques.