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Topic: MS-MS of peptide (subtracting peaks to get molecular weight of amino acid)  (Read 2186 times)

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Offline Moz29386

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MS-MS of peptide (subtracting peaks to get molecular weight of amino acid)
« on: February 09, 2012, 02:12:49 PM »
Good evening folks.

I am trying to sequence the amino acids present in this tandem MS-MS spectrum:



I have tried labelling the Y and B ions but failed miserably. I know the principle lies behind substracting say y7 - y6 = to give molecular weight of one amino acid and so on...

I don't know where to start really.

Any help is greatly appreciated.
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Offline Moz29386

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Re: MS-MS of peptide (subtracting peaks to get molecular weight of amino acid)
« Reply #1 on: February 09, 2012, 02:57:06 PM »
Oh and peptide sequence is CF-Gly-Leu-Ala-Asp-Lys-Leu-Tyr-Ser

CF is carboxyfluorescein buts thats not really relevant atm.
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