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Topic: possible to extract DNA by freezing?  (Read 3840 times)

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Offline NathanielZhu

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possible to extract DNA by freezing?
« on: March 30, 2012, 10:21:28 PM »
Ok, so I froze my bottle of cranberry juice and I was sucking on it for a while and after a while, I noticed sticky red (from the color of the cranberry) mucus-like stuff attached from the ice to the wall of the bottle.
It could be some of my saliva (though my saliva isn't as sticky as this stuff, nor does saliva leave any mass behind after dehydrating it) so I'm thinking maybe it's dna.

What do you guys think?

Offline Babcock_Hall

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Re: possible to extract DNA by freezing?
« Reply #1 on: April 02, 2012, 10:28:30 AM »
My guess is that DNA would not be that voluminous.  What about material from the plant cell wall as a possibility?

Offline fledarmus

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Re: possible to extract DNA by freezing?
« Reply #2 on: April 02, 2012, 01:21:19 PM »
I agree with Babcock Hall - I don't think you will get much DNA from either your cranberry juice or your saliva, and I doubt it can be separated by freezing. I suspect you have pectin from the skins of the cranberries. This is a polysaccharide found in the cell walls of plants and is the component in fruit that gells to form jellies and jams. Cranberries are high in pectin.

genetic1

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Re: possible to extract DNA by freezing?
« Reply #3 on: April 04, 2012, 01:49:53 AM »
If you freeze anything that contains water, the water molecules that are normally randomly in motion because water is liquid will become fixed in a crystalline. This forms ice that is larger in volume than the original liquid volume. These ice crystals will tear and puncture anything in the vicinity including large molecules such as DNA. The DNA can still be extracted but it cannot be guaranteed to be intact. You could try to freeze it in DMSO to avoid the ice crystals. I do remember something about fungal DNA extraction using freezing method before but I could be wrong. I would avoid using cranberry juice because it does contain water especially if your buying it from concentrate from the store. simply because there will not be much DNA intact afterwards.

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