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Topic: Dilution Calculation  (Read 16355 times)

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Offline ziagemini

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Dilution Calculation
« on: April 27, 2012, 02:25:44 AM »
Hallo All,

I would like to ask a question regarding the dilutions. I have 10 grams of a sample. I would like to dilute it in 1:10 ratio. What i have to do:
1. Put 10g in a flask and fill it till 100ml mark with DW?
2. Or put 10g in a flask and add 100ml of DW?

Secondly from my diluted concentration how will i get the original mass or concentration? If i don't know its 10 grams?

Hope to hear soon. Thanks in advance.

Regards!

Offline AWK

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Re: Dilution Calculation
« Reply #1 on: April 27, 2012, 03:16:18 AM »
Just add 100 g of water
« Last Edit: April 27, 2012, 04:33:34 AM by AWK »
AWK

Offline ziagemini

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Re: Dilution Calculation
« Reply #2 on: April 27, 2012, 05:17:30 AM »
Hello,

It means 100ml. As the density of water is 1000. So it does'nt matter i add 100grams or 100ml.(but most of the places from sample prepration its always written fill upto 100ml mark not add 100ml water)

That means, 2. is the right one. So that also means if i have 9grams and tried to reach 1:10 dilution then adding 90ml or 90 grams i reach my desired dilution.

Secondly what about the reverse? If i have a sample of sludge(containing acetic acid) and given that it is diluted in the ratio of 1:10. I find the concentration using any techniques lets say Titration and got 0.1mol/L concentration. so i have 60.05g/L but its with dilution included what if i want to find the actual mass from it. How i will remove the dilution factor from it.

Hope to hear soon. Thanks.

Regards!

Offline Twickel

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Re: Dilution Calculation
« Reply #3 on: April 27, 2012, 08:32:59 AM »
I thought that 1:10 means that, there are a total of ten parts.
So the 10g of your solute is 1 part to 9 parts solvent.

So you should only be adding 90mL of water.

Offline fledarmus

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Re: Dilution Calculation
« Reply #4 on: April 27, 2012, 08:55:39 AM »
This is a completely ambiguous question and I wish teachers and test-makers would stop writing questions like this. The answer depends entirely on the context of the problem. What do you intend to do with the solution, and what are you trying to measure?

If you are working with a procedure that requires you to weigh out your final material, a 1:10 dilution is designed to give you a 10% w/w (weight-to-weight) final solution. This means that when you weigh out 10 grams of your final solution, it will contain 1 gram of your starting component. In that case, you would use Twickels formulation - add 90 g water.

If you are working with a procedure that requires you to measure out your final solution by volume, a 1:10 dilution is designed to give you a 10% w/v (weight-to-volume) final solution. This means that when you measure out 10 mL of your final solution, it will contain 1 gram of your starting component. In that case, you would your proposed answer number 1 - put it in a volumetric flask and dilute it to 100 mL of total solution (fill to 100 mL mark). Since your question about reversing the calculations is using volumes, my guess is that this is actually the procedure you need to be using.

To do the calculations in reverse, you would just use the relationship that 10 mL of your test solution contained 1 gram of your initial solution.

Offline AWK

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Re: Dilution Calculation
« Reply #5 on: April 27, 2012, 12:34:16 PM »
I thought that 1:10 means that, there are a total of ten parts.
So the 10g of your solute is 1 part to 9 parts solvent.

So you should only be adding 90mL of water.
Dilution 1:10 literally means 10 g of solute and 100 g of solvent.
Dilution 10 g of solute and 90 g(ml if water is a solvent) means  "ten times"
AWK

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