[ciel]

Hello,


could anyone explain why the reversed-phase HPLC is more common used nowadays compared to the normal phase?

Aren't they the same? From what I read, the difference is only that, in the normal phase, the compound that will come out first from the column is the most non-polar one, while in the reversed phase, the first eluted compound is the most polar compound. Is this correct?

Thanks

[Albert]

Reversed-phase HPLC is widely used beacause it has less problems for what concerns the pollution of the column, compared to normal-phase HPLC.

Moreover, it can be used to analyse drugs (I did it), amino acids and peptides.
In non-aqueous form, it gives you the opportunity to separate fats.

[jwesterway]

"Normal phase" refers to a np solvent mobile phase is put through a polar stationary phase.
"Reverse phase" is the opposite, a polar solvent mobile with a np stationary.

-Josh