[raydubbs]

You perform column chromatography on a mixture of reaction products which show two spots on TLC. However, under the column conditions you chose those two compounds do not separate. Decide whether you should increase or decrease the following factors to improve the resolution:
column diameter (decrease), column length (increase), sample volume (decrease), sample concentration (decrease), loading time (?), flow rate (decrease).

I'm confused about what loading time has to do with it?

[Arkcon]

So I'm left guessing that the column media and eluent are exactly the same as the TLC plate media and developing solvent.  That's hard to achieve, but, whatever.  So it seems changing conditions isn't an option.  The tiny amount spotted and driven by capillary action separates, but the amount loaded on the column won't.  So what effect do your options have on your separation?