[t1bbst3r]

Soz chaps,
                  I have just 1 question then I'm free.

If you extract a powder with a liquid & decant the supernatant you seem to be removing the waste products in the liquid don't you.

Now, if you then further fractionate the residue in more liquids (acetone) on a Sephadex LH-20 column to get 1/100th of the original residue, just what are you doing by this?

(In response to my last post, this is'nt a patent violation, if thats going to matter, because you can't patent this sort of stuff, I looked it up, its easy to obtain & will always be also free, but you can patent a drug made up of many components if you wish, so something like the 'active-ingredient' of asprin isn't an intellectual property right if that makes sense (just the brand name). Hopefully thats any stigma I may otherwise get over & done with now anyway)

Sure hope somebody can answer my question. CHEERS!

[Arkcon]

Soz chaps,
                  I have just 1 question then I'm free.

If you extract a powder with a liquid & decant the supernatant you seem to be removing the waste products in the liquid don't you.

You possibly have the terms backward -- extracting from a solid and decanting is usually leaving behind unwanted products in the solid.  But you seem to get it correct later.

Now, if you then further fractionate the residue in more liquids (acetone) on a Sephadex LH-20 column to get 1/100th of the original residue, just what are you doing by this?

Sephadex LH-20 is solvent compatible type of size exclusion media.  Molecules that are "bigger" travel faster down the column than smaller ones.  The sizes are often comparable to molecular weight, but not absolutely.  At any rate, dilution isn't what's happening, its a way of separating impurities based on molecular weight.

(In response to my last post, this is'nt a patent violation, if thats going to matter, because you can't patent this sort of stuff, I looked it up, its easy to obtain & will always be also free, but you can patent a drug made up of many components if you wish, so something like the 'active-ingredient' of asprin isn't an intellectual property right if that makes sense (just the brand name). Hopefully thats any stigma I may otherwise get over & done with now anyway)

Sure hope somebody can answer my question. CHEERS!

uhh ... yes?