[pantone159]

Why, exactly, would meat tenderizer be used?  I have seen some procedures on the web that call for this.  They say something about removing proteins that the DNA is attached to.  Would using Na acetate have the same effect?

[bryanh]

I think meat tenderizer is indeed used to degrade proteins that the DNA is bound to, and possibly to degrade cellular membrane proteins too. Both kiwi fruit and pineapples contain a similar enzyme (why we put pineapple on gammon steak) and DNA can be more easily extracted from them.
There are many different ways to extract DNA depending on what your getting it from and you want in terms of purity etc. For someone wanting to extract DNA at home the question is why they want to do this. If its just for some fun and to impress the chicks (in case you can't tell im being sarcastic) then there are very simple recipes on line to extract DNA from kiwi, you can actually see and pick up the long stringy molecule.

If your trying to extract your own DNA to clone yourself or build an army of supersoldiers or something then things get more precise and i'm not sure i'd like to help anyone out on this. 

[pantone159]

If your trying to extract your own DNA to clone yourself or build an army of supersoldiers or something then things get more precise and i'm not sure i'd like to help anyone out on this. 

I'm just trying to clone some of the pretty ladies.  I don't have the patience to wait decades for them to grow up, so I am kind of stumped on that point.   

[Donaldson Tan]

I don't have the patience to wait decades for them to grow up

I don't think this will be a problem if you are a paedophile. LOL.

[2810713]

I'm just trying to clone some of the pretty ladies.  I don't have the patience to wait decades for them to grow up, so I am kind of stumped on that point.   

Use hybridoma-like technology! Its fast! Believe me... & I think u take face from actress A , lips of B, Legs of C, and [ i think , i should stop here...]

& One confirmation that why the pineapple enzyme doesn't kill its own proteins is as the enzyme is in the vescicles and not free... BUt when we add soap it comes out, right?

[caramon84]

So anyone can help...what happen if water is added instead of alocohol and why?

[Yggdrasil]

If you try to precipitate DNA with water instead of alcohol, it won't work.  DNA is soluble in water.

[sdekivit]

So anyone can help...what happen if water is added instead of alocohol and why?

in order to precipitate the DNA you need to get rid of the water that interacts with the DNA.

--> water thus has no effect (note that the phosphate backbone of DNA is negatively charged !)