September 27, 2024, 05:33:59 PM
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Topic: Trouble with Spectrophotometry  (Read 2933 times)

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Offline BetterCallSaul

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Trouble with Spectrophotometry
« on: December 27, 2013, 05:19:06 PM »
I can't seem to find any data for the absorption coefficients of the Nickel(2+) ion at wavelengths of 530nm, 578nm and 640nm to be  used for the  Beer-Lambert law. If anyone has any websites where I can find this information to share it  would be  greatly appreciated.

Regards,
A clueless Chemistry Undergraduate

Offline marquis

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Re: Trouble with Spectrophotometry
« Reply #1 on: December 29, 2013, 11:49:12 AM »

I've always seen some variation in coefficients, depending on the instrument
used and parameters selected.

Usually, the coefficients are calculated by running known standards and then
applying Beers-Lambert to calculate. 

Is this possible?  Do you have access to an instrument?

Good luck.

Offline Corribus

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Re: Trouble with Spectrophotometry
« Reply #2 on: December 29, 2013, 08:58:05 PM »
The extinction coefficient will depend on the ligands.
What men are poets who can speak of Jupiter if he were like a man, but if he is an immense spinning sphere of methane and ammonia must be silent?  - Richard P. Feynman

Offline BetterCallSaul

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Re: Trouble with Spectrophotometry
« Reply #3 on: December 30, 2013, 09:18:41 AM »

Usually, the coefficients are calculated by running known standards and then
applying Beers-Lambert to calculate. 


Ah, yes that helps a lot as in my  results I have a Mole fraction of 0 for NiS04.
The equation they have given me is Y [ = Ameasured - (1-x)AM ]

Am I right is saying that this means that at a mole fraction of 0, the measured absorbance value will be my extinction coefficient (AM for that wavelength?

Thanks a lot  for the help.

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