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Topic: Sodium Phosphate Buffer  (Read 8824 times)

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gsbhat03

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Sodium Phosphate Buffer
« on: March 08, 2006, 11:52:05 PM »
Hi

Anybody Knows how to prepare sodium phosphate buffer of pH of 5.5 I am getting ony pH more than 5.7??
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Offline AWK

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Re:Sodium Phosphate Buffer
« Reply #1 on: March 09, 2006, 01:23:56 AM »
It is rather a wrong idea. Phosphate buffers can work effectively within pH 1.2-3.2 or 6.2-8.2
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Offline Borek

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Re:Sodium Phosphate Buffer
« Reply #2 on: March 09, 2006, 03:18:56 AM »
Go for citric buffer.
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savoy7

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Re:Sodium Phosphate Buffer
« Reply #3 on: March 09, 2006, 10:43:06 PM »
I've used Sodium Phosphate Buffers from 6 to 7.4.

If you go with Borek's suggestion:


0.1 M Citrate Buffer at pH 5.5
9.6 g of anhydrous citric acid & 4.4 g of NaOH.  Dilute to 500 ml.  Adjust pH with NaOH ( maybe a 10M ).

just a suggestion
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