[ikariotis]

Hi all!

I try to find enzymes which contains tyrosine in active site. I've been seaching but cant find enzymes list containing tyrosine in active site and their corresponing reaction mechanisms (in structural formulas). plese help me!

[zoork34]

BRENDA is a useful enzyme database.  Just searching for tyrosine brings up a few possible hits such as:

http://www.brenda-enzymes.org/php/result_flat.php4?ecno=1.11.1.6

[Babcock_Hall]

Tyrosine residues can have some surprising chemistry.  Some enzymes have a radical on the oxygen of tyrosine in the resting state.

[ikariotis]

I know that tyrosine can act as nucleophile/electorphile, be apart of phoshorylation reaction, radical intermediates, and covalent binding in enzyme reactions. The problem is that a have to find different enzymes which have the tyrosine represented in active site and also show the detailed reaction mechanism in strucutral formulas. I used brenda but cant understand how to do the seach specific for tyrosine containing active sites? To find the reaction mechanism is also difficult. I´ve search on google, brenda, uniprot ect...maybe i just now the different fucntions of the site for finding this request.

[Babcock_Hall]

One might search for enzymes for which tyrosine is essential, although I am not sure exactly how one might do this (a Pubmed search using "tyrosine" and "essential" as search terms, or "tyrosyl radical" or "tyrosyl intermediate").  Another approach is to start anecdotally:  work with enzymes where you know the function of tyrosine and search for their mechanism.  For example there is one type of ribonucleotide reductase that has a tyrosyl radical, and you could search for papers on its mechanism.  I would also use textbooks that are specific for enzyme reaction mechanisms, such as one coauthored by Perry Frey.  Have you found any enzymes so far?  There is at least one enzyme in DNA metabolism that uses tyrosine as the nucleophile to form an intermediate.

[ikariotis]

Thanks! I will try that book , very good advice