[techlab]

Hello everybody I'm newbie here and my native language is not English.
So I'm sorry of my bad language.
My problem is how to dilute toluene. I have to calculate count plate and resolution factor
Agilent 1100 HPLC device.

I have to make sample which contains:
Uracil 5 µg/ml
Phenol 200 µg/ml
4- Chloronitrobenzene 25 µg/ml
Toluene 850 µg/ml

This sample have dilute to 80/20 methanol/water (v/v)
I have to make liquid 500 ml

It's easy to weigh out solid reagents (uracil, phenol and 4-Chloronitrobenzene )
using formula c = m x V
But how I dilute toluene. It's liquid and I cant use anymore c = m x V.
I have to use density to calculate toluene amount but how.

Can somebody help.

Here is Agilent data sheet: http://www.chem.agilent.com/Library/specifications/Public/820114-002.pdf

[sjb]

Hello everybody I'm newbie here and my native language is not English.
So I'm sorry of my bad language.
My problem is how to dilute toluene. I have to calculate count plate and resolution factor
Agilent 1100 HPLC device.

I have to make sample which contains:
Uracil 5 µg/ml
Phenol 200 µg/ml
4- Chloronitrobenzene 25 µg/ml
Toluene 850 µg/ml

This sample have dilute to 80/20 methanol/water (v/v)
I have to make liquid 500 ml

It's easy to weigh out solid reagents (uracil, phenol and 4-Chloronitrobenzene )
using formula c = m x V
But how I dilute toluene. It's liquid and I cant use anymore c = m x V.
I have to use density to calculate toluene amount but how.

Can somebody help.

Here is Agilent data sheet: http://www.chem.agilent.com/Library/specifications/Public/820114-002.pdf

You can still weigh out liquids, or alternatively what is the definition of density?

[techlab]

I think I cannot weight it because toluene density is 0.87 g/mL (20 °C).

[Hunter2]

Why not. 850 µg correspond to how many µl?

[techlab]

850 µg correspond 850 µl if you use water because it's density is 1
but toluene's density is 0.87 g/ml.

[Hunter2]

And whats the problem now. Calculate the volume by using the density.

[techlab]

And whats the problem now. Calculate the volume by using the density.

Thats the problem.

[Hunter2]

What is the definition of density or specific gravity. Hint check the unit.