[Babcock_Hall]

I don't have much experience working with proteins and detergents, other than SDS.  Do people routinely make up stock solutions in the range of 1-10%?  How do they store them in a way that minimizes the formation of peroxides?  I would think dark glass or plastic would be good, and possibly storing in the presence of nitrogen gas would make sense.  But I don't have a good notion of how quickly peroxides form or how effective the methods for detecting and eliminating them are.  We plan to work with Triton X-100 at 0.015% concentration, and we sometimes work with various polyethylene glycols (PEGs) at much higher concentrations.  Here is what I have found so far on the formation of peroxides:

1.   A simple procedure for removing contaminating aldehydes and peroxides from aqueous solutions of polyethylene glycols and of nonionic detergents that are based on the polyoxyethylene linkage.
Ray WJ Jr, Puvathingal JM.
Anal Biochem. 1985 May 1;146(2):307-12.
PMID: 4025798 [PubMed - indexed for MEDLINE]

2.   Peroxide accumulation in detergents.
Jaeger J, Sorensen K, Wolff SP.
J Biochem Biophys Methods. 1994 Jul;29(1):77-81.
PMID: 7989648 [PubMed - indexed for MEDLINE]

3.   Highly reactive impurities in Triton X-100 and Brij 35: partial characterization and removal.
Ashani Y, Catravas GN.
Anal Biochem. 1980 Nov 15;109(1):55-62. No abstract available.
PMID: 7469018 [PubMed - indexed for MEDLINE]

4.   Peroxides in detergents as interfering factors in biochemical analysis.
Lever M.
Anal Biochem. 1977 Nov;83(1):274-84. No abstract available.
PMID: 920946 [PubMed - indexed for MEDLINE]

[Jefke]

Good question.
Anyone an idea about the long term storage of SDS stock solutions?

[Babcock_Hall]

I have never heard of a tendency to form peroxides in solutions of SDS, but you raise an interesting point.

[Jefke]

I have never heard of a tendency to form peroxides in solutions of SDS, but you raise an interesting point.

I found one paper that speaks of the stability of SDS , but not sure it is what you are looking for.
THe paper is this one: On the Stability of Aqueous Sodium Dodecyl Sulfate Solutions
(link: http://www.sciencedirect.com/science/article/pii/S0021979783714293)
To me it seems that it is rather stable and ok to make a stock solution and keep it for years.