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Topic: Ninhydrin test turned orange then blue?!  (Read 1474 times)

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Offline Karens

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Ninhydrin test turned orange then blue?!
« on: August 06, 2014, 11:19:54 AM »
When synthesizing a peptide sequence KLTWQELYQLKYKGI, my ninhydrin test turned orange than blue when I was testing for deprotection. Something wrong? ???
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Offline Babcock_Hall

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Re: Ninhydrin test turned orange then blue?!
« Reply #1 on: August 06, 2014, 04:20:18 PM »
I don't recall seeing exactly that sequence of events.  However, I have noticed that when spotting an amine very heavily, the color is not the same as when the same substance is spotted lightly.
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