January 09, 2025, 01:51:20 PM
Forum Rules: Read This Before Posting


Topic: Colorimetry Calibration graph  (Read 2438 times)

0 Members and 1 Guest are viewing this topic.

Offline chickapow

  • Regular Member
  • ***
  • Posts: 18
  • Mole Snacks: +0/-0
Colorimetry Calibration graph
« on: March 12, 2015, 02:56:03 PM »
What best fitting line should I plot on my graph? Linear? Polynomial? Logarithmic?

Offline Borek

  • Mr. pH
  • Administrator
  • Deity Member
  • *
  • Posts: 27894
  • Mole Snacks: +1816/-412
  • Gender: Male
  • I am known to be occasionally wrong.
    • Chembuddy
Re: Colorimetry Calibration graph
« Reply #1 on: March 12, 2015, 03:05:50 PM »
Graph of what vs what?
ChemBuddy chemical calculators - stoichiometry, pH, concentration, buffer preparation, titrations.info

Offline chickapow

  • Regular Member
  • ***
  • Posts: 18
  • Mole Snacks: +0/-0
Re: Colorimetry Calibration graph
« Reply #2 on: March 12, 2015, 03:07:08 PM »
Graph of what vs what?

Sorry, should have specified;

Concentration against absorption.

Offline Corribus

  • Chemist
  • Sr. Member
  • *
  • Posts: 3554
  • Mole Snacks: +546/-23
  • Gender: Male
  • A lover of spectroscopy and chocolate.
Re: Colorimetry Calibration graph
« Reply #3 on: March 12, 2015, 03:23:02 PM »
If you're doing a UV-Vis expt, Beer's Law specifies a linear relationship, so you should be fitting to a line. Unless you are in a range where Beer's Law breaks down, in which case you should be fitting to something else. If you're doing a concentration curve, you should be in the linear range, and should be fitting accordingly, although some instrument software allows you to incorporate curvature into a fit.
What men are poets who can speak of Jupiter if he were like a man, but if he is an immense spinning sphere of methane and ammonia must be silent?  - Richard P. Feynman

Offline chickapow

  • Regular Member
  • ***
  • Posts: 18
  • Mole Snacks: +0/-0
Re: Colorimetry Calibration graph
« Reply #4 on: March 12, 2015, 08:33:41 PM »
If you're doing a UV-Vis expt, Beer's Law specifies a linear relationship, so you should be fitting to a line. Unless you are in a range where Beer's Law breaks down, in which case you should be fitting to something else. If you're doing a concentration curve, you should be in the linear range, and should be fitting accordingly, although some instrument software allows you to incorporate curvature into a fit.

Ah ok, thank you : )

Sponsored Links