[pharmacist1]

Hi friends, can anybody help me in this question. how to prepare buffer solution of pH 5.4?

[thetada]

Happy to but it's forum policy that you show what you've managed to come up with by yourself so far.

[Arkcon]

I'd like to welcome you, pharmacist1: to the Chemical Forums.  According to the Forum Rules{click}, we want to see your attempt, so please click on that link, read the contents, and comply with our rules, so we can help you. 

Are you aware of a formula that defines pH of buffers, its a pretty common one in elementary chemistry.  Once you have that formula, you'll know what else you'll need.  You will have to, at the very least, select what buffer is compatible with the work you want to do.