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Conversion of Ethanol to Acetaldehyde with H202 and Catalase
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Topic: Conversion of Ethanol to Acetaldehyde with H202 and Catalase (Read 2744 times)
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WTK28
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Conversion of Ethanol to Acetaldehyde with H202 and Catalase
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August 18, 2015, 11:49:29 AM »
So I am trying to determine how much H202 and Catalase I need to combine with ethanol to completely convert all the ethanol to Acetaldehyde without leaving excess H202 and Catalase unreacted. Any ideas?
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Babcock_Hall
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Re: Conversion of Ethanol to Acetaldehyde with H202 and Catalase
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Reply #1 on:
August 18, 2015, 12:13:33 PM »
Is catalase a reactant or a catalyst? My first thought is to ask whether or not you are sure that ethanol is a substrate for catalase. My second thought is to suggest that you look into alcohol oxidase.
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WTK28
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Re: Conversion of Ethanol to Acetaldehyde with H202 and Catalase
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August 18, 2015, 01:43:12 PM »
In short. Catalyst.
I am trying to understand how ethanol is converted to acetaldehyde in the peroxisomes of the cell using catalase protein and H2O2, and then replicate that experiment in vitro.
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Babcock_Hall
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Re: Conversion of Ethanol to Acetaldehyde with H202 and Catalase
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August 18, 2015, 02:15:03 PM »
Catalase converts hydrogen peroxide into water and oxygen. I suspect that a different enzyme is responsible for oxidizing ethanol, but I don't have detailed knowledge of the peroxisomes.
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Conversion of Ethanol to Acetaldehyde with H202 and Catalase