[cockleivory]

In our lab report for immobilized metal affinity chromatography (IMAC), we are asked to compare HiTrap affinity column (chelating group is iminodiacetic acid, IDA), the NTA His-Bind column and the NDA-agarose column.

I have problem
(1) finding information about the NDA-agarose column
(2) selecting characteristics of the column to compare (I've found a paper that says Ni2+-NTA is more stable than Ni2+-IDA and that it selects for neighboring histidine molecules, but that's all I have right now)

Could anybody give any hints as to where to look for more info?
p.s. I did not learn anything about coordination and transition metal so it's hard for me to understand the principles behind IMAC.

[Babcock_Hall]

With respect to chromatography in general, can you think of desirable properties for a stationary phase to have?

[Babcock_Hall]

One factor to think about is capacity.

[cockleivory]

Like the capacity factor or retention factor, and height equivalent to a theoretical plate?
But don't I also need info about the mobile phase for all these? What if the three column products suitable for separation of different molecules?

[Babcock_Hall]

Capacity is essentially unrelated to the composition of the mobile phase.  I don't understand you last question.