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Topic: Lycopene recovered on a wet weight and dry weight basis?  (Read 2143 times)

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Offline HelloMoto

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Lycopene recovered on a wet weight and dry weight basis?
« on: March 08, 2016, 12:05:09 AM »
We dehydrated some tomato paste using acetone and dichloromethane and left the filtrate to evaporate in a warm water bath.

I recovered about 0.009g of Lycopene product from the original 35g of tomato paste.

I'm asked to express the amount of lycopene product recovered as:

mg product per gram of raw material on a wet weight basis
mg product per gram of raw material on a dry weight basis

I'm lost as to what wet weight and dry weight are. If you need more information, please let me know, and thanks in advance.

Offline Arkcon

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Re: Lycopene recovered on a wet weight and dry weight basis?
« Reply #1 on: March 08, 2016, 06:12:37 AM »
It really depends on how the lab manual wants to define the terms.  I don't see how you "dehydrated" using a pair of solvents.  In order to determine mg of product per wet and dry weight you'll need to have weighed something, both wet and dry, but you seem to be short a weighing.

Anyway, you have a weight of lycopene and a wight of raw material, calculate mg product per gram of raw material.  At least you've done some work you can report.  The problem now is which of the two answers is it, and how will you get the other one.
Hey, I'm not judging.  I just like to shoot straight.  I'm a man of science.

Offline Babcock_Hall

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Re: Lycopene recovered on a wet weight and dry weight basis?
« Reply #2 on: March 08, 2016, 08:50:26 PM »
The wet and dry might refer to the original tomato paste, not the lycopene.  BTW did you do anything to remove phospholipids and other material that you extracted (assuming that this was an extraction)?  Just curious; I used to work with carotenoids.

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