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Topic: Phophatase activity  (Read 5392 times)

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Ness

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Phophatase activity
« on: May 19, 2006, 12:47:16 PM »
I have a problem with a tyrosine phophatase assay. First of all the standards all turn blue the same way with malachite green, and secondly some of the standards and samples produce a precipitate with the malachite green solution and molybdic acid. I have hydrocortisone hemisuccinate in some samples, and they all produce a precipitate. I don't have a clue about chemistry as I am a medical student so please!!!! help me!!!! ???  ???
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Ness

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Re: Phophatase activity
« Reply #1 on: May 19, 2006, 12:48:43 PM »
I ment phosphatase activity ...
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Offline edimerz

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Re: Phophatase activity
« Reply #2 on: May 21, 2006, 02:04:50 AM »
Hi

Can U please explain a bit about how are you isolating your tyrosine kinase - recombinant or extracted?
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Offline Equi

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Re: Phophatase activity
« Reply #3 on: May 21, 2006, 06:31:12 AM »
Detailed essay conditions?
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