You have much more work to do before you can consider this done. Like Borek: said, you haven't told us the properties of A, B, C, or D. I don't know why you specify 4 ml of solvent, without telling us how big the "pellet" is. Example, You will not find 1 gram of sucrose sealed in a 10g ball of iron-nickel-chromium alloy with 4 mL of water. And I chose sucrose for a double incorrect answer -- since it doesn't have much UV absorbance. You haven't specified possible ranges for unknown, and how you will bracket with standards. There are a number of non-ideal situations that may cause the plan to fail. But in general, yes, a calibration curve of pure material will help.