[parisa-fateh]

Can i make 100mM acetate buffer and the dilute it to reach 64mM?

[Babcock_Hall]

Yes, there is a simple formula that is based on the idea that the amount of any chemical species (typically expressed in moles) is conserved.  Are you familiar with it?

[parisa-fateh]

I found this table to prepare acetate buffer (0.1 M).
http://delloyd.50megs.com/moreinfo/buffers2.html
Should I prepare this concentration and the diluted it with distilled water?
Also I would like to know how long the buffer solution will be stable?

[Borek]

You have ignored the question: do you know the formula used for buffer preparation? Calculating required amounts of acetate and acetic acid is quite a simple task, actually you are overcomplicating it by searching for ready recipes.

[parisa-fateh]

actually I don't know how I can calculate it by myself!

[Arkcon]

actually I don't know how I can calculate it by myself!

Hi there, parisa-fateh:, I'd like to welcome you to the Chemical Forums, and you've been here for a little while, but I want to ask you to review our Forum Rules{click}.  You already accepted the rules when you signed up for our forum, and you have to follow them, whether you agree with them or not, or even if you're unaware of them.

We want to see you do some work, on your own, and we'll help.  We don't dump complete answers, for anyone, not for a high school student, nor a hard-working professional, nor even the home chemist working on their newest invention.

Let's try to think about your question:

Can i make 100mM acetate buffer and the dilute it to reach 64mM?

Think about this:  You have 1 gram of any salt in a liter of water.  You add another liter of water.  Now how many grams of salt do you have per liter?

This is simple math.  Molarity follows the same dilution rules*.  This isn't chemistry, this is sub-high school math.  Most of your questions involve nanotechnology and advanced concepts, but that doiesn't absolve you from doing your own math.  Or at least trying to.

*However .

You've left off the questions if the pH will stay the same with dilution.  Or if ionic strength will track linearly.  It might not, so we're dubious about just dumping you answer, that may turn out to be wrong once you add qualifiers.

Also I would like to know how long the buffer solution will be stable?

This topic comes up often on these boards.  And there is no really good answer.  And we've mentioned in other threads why.  Do yourself a favor, and browse and search the Chemical forums for similar questions.  You may find all the answers you need.