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Topic: Optimisation of elution conditions using designed experiments in HPLC  (Read 1250 times)

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Offline Legolasas5

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Optimisation of elution conditions using designed experiments in HPLC
« on: February 20, 2019, 04:30:15 PM »
Hello there,
I am trying to optimise HPLC method and I have a problem with 2 peaks that change their selectivity depending on elution conditions. Is it appropriate to have a negative resolution as a response in the DoE table if these peaks switch.
Thank you for your answers.
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